FRET is based on the dipole-dipole coupling mechanism, where the energy transfer occurs through non-radiative means. It is highly efficient over short distances and sensitive to the separation between ...

Time-resolved fluorescence resonance energy transfer (TR-FRET) is a powerful tool for drug discovery, enabling the analysis of molecular interactions in a wide variety of biochemical processes. This ...

To understand biochemical reactions, measurement of stoichiometry is crucial. This is because these reactions depend on the interaction of at least two cellular components. Measuring these ...

To characterize enzymatic activity of severe acute respiratory syndrome (SARS) coronavirus (CoV) 3C-like protease (3CL pro) and its four site-directed mutants. Based on the fluorescence resonance ...